Coding

Part:BBa_J70631

Designed by: Julie Norville   Group: Knight Lab   (2010-08-21)


R0010-J70630, rbs and maltose binding protein added to J70423

R0010-RBS-MBP-(E0040-start sequence)

J70630 was entered separately because it contains the part of the sequence that does not contain scars

J70630 describes how the RBS MBP was made

however, basically J70423 was cut with PsrI, mbp was amplified with primers that contained the rbs and then was cut with PsrI and inserted into cut J70423

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 602
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 300
    Illegal BsaI.rc site found at 1990


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Categories
Parameters
None